fibroblast migration murine fibroblasts Search Results


dmem  (FUJIFILM)
90
FUJIFILM dmem
Dmem, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fibroblast+migration+murine+fibroblasts/dmem/pmc03573719-42-7-15
Average 90 stars, based on 1 article reviews
dmem - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
recombinant mouse fgf basic/fgf2/bfgf protein  (Bio-Techne corporation)
95
Bio-Techne corporation recombinant mouse fgf basic/fgf2/bfgf protein
Recombinant Mouse Fgf Basic/Fgf2/Bfgf Protein, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fibroblast+migration+murine+fibroblasts/Recombinant+Mouse+FGF+basic%2FFGF2%2FbFGF+Protein/bio-techne+corporation___3139-fb
Average 95 stars, based on 1 article reviews
recombinant mouse fgf basic/fgf2/bfgf protein - by Bioz Stars, 2026-09
95/100 stars
  Buy from Supplier
murine colon cancer cell line ct26  (ATCC)
99
ATCC murine colon cancer cell line ct26
Murine Colon Cancer Cell Line Ct26, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fibroblast+migration+murine+fibroblasts/CT26%2EWT/pmc10293063-90-1-10
Average 99 stars, based on 1 article reviews
murine colon cancer cell line ct26 - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
primary murine lung fibroblasts  (Helmholtz Zentrum fur Infektionsforschung GmbH)
90
Helmholtz Zentrum fur Infektionsforschung GmbH primary murine lung fibroblasts
Primary Murine Lung Fibroblasts, supplied by Helmholtz Zentrum fur Infektionsforschung GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fibroblast+migration+murine+fibroblasts/primary+murine+lung+fibroblasts/10__1128_slash_jvi__01524___14-250-22-10
Average 90 stars, based on 1 article reviews
primary murine lung fibroblasts - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
gene exp ifnb1 mm00439552 s1  (Thermo Fisher)
99
Thermo Fisher gene exp ifnb1 mm00439552 s1
Intraperitoneal administration of nitroalkene compound CP-36 dampens STING-dependent inflammation in vivo. (A–C) Mice were administrated with CP-36 i.p. (10 or 30 mg/kg) 2 h before activating STING with i.p. injection of cAIM(PS) 2 Difluor (Rp/Sp) (0.2 mg/kg) for 4 h. Immunoblot analysis of homogenized and lysed (A) lung and (B) liver tissue to evaluate the levels of pTBK1, TBK1, pSTAT1, STAT1 and Viperin with (C) densitometry quantification of protein levels. The plots include data from individual mice represented in immunoblots. Bars indicate mean ± SEM and are normalized to the cAIM(PS) 2 Difluor (Rp/Sp) group, expressed as a percentage. (D–F) WT or STING-KO BMDMs were pre-treated with CP-36 (5 and 10 μM) or reduced CP-36 (R-CP-36, 5 and 10 μM) for 2 h, followed by cGAMP stimulation (4 μg/mL) for either 3 h or 6 h. (D) Immunoblot analysis of whole-cell lysate (representative blot) to evaluate levels of pSTING, STING, pIRF3 and IRF3 and (E) densitometry quantification of pSTING/STING protein levels after 3 h cGAMP stimulation. Three independent experiments are plotted. (F) qPCR analysis of <t>Ifnb1</t> and Cxcl-10 expression was quantified relative to 18S mRNA levels after 6 h of cGAMP stimulation. BMDM cultures obtained from 6 individual mice (3 male and 3 female mice) are plotted. Bars indicate mean ± SEM and are normalized to cGAMP levels, expressed as a percentage. Statistical analyses were performed using Welch's t -test, yielding the following p values: For tissues: pTBK1: p = 0.0293, TBK1: p = 0.0283, Viperin: p = 0.0311, STAT1: p = 0.0159. For BMDMs: pSTING/STING: p = 0.0184, Ifnb1 : p < 0.0001, Cxcl-10 : *, p = 0.0367 and **, p = 0.0076. * Indicates p < 0.05, ** indicates p < 0.01, and **** indicates p < 0.0001.
Gene Exp Ifnb1 Mm00439552 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fibroblast+migration+murine+fibroblasts/Gene+Exp%2E+Ifnb1%2C+Mm00439552_s1/pmc11215336-333-5--1
Average 99 stars, based on 1 article reviews
gene exp ifnb1 mm00439552 s1 - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
murine embryonic fibroblasts nih 3t3 cells  (ATCC)
99
ATCC murine embryonic fibroblasts nih 3t3 cells
Intraperitoneal administration of nitroalkene compound CP-36 dampens STING-dependent inflammation in vivo. (A–C) Mice were administrated with CP-36 i.p. (10 or 30 mg/kg) 2 h before activating STING with i.p. injection of cAIM(PS) 2 Difluor (Rp/Sp) (0.2 mg/kg) for 4 h. Immunoblot analysis of homogenized and lysed (A) lung and (B) liver tissue to evaluate the levels of pTBK1, TBK1, pSTAT1, STAT1 and Viperin with (C) densitometry quantification of protein levels. The plots include data from individual mice represented in immunoblots. Bars indicate mean ± SEM and are normalized to the cAIM(PS) 2 Difluor (Rp/Sp) group, expressed as a percentage. (D–F) WT or STING-KO BMDMs were pre-treated with CP-36 (5 and 10 μM) or reduced CP-36 (R-CP-36, 5 and 10 μM) for 2 h, followed by cGAMP stimulation (4 μg/mL) for either 3 h or 6 h. (D) Immunoblot analysis of whole-cell lysate (representative blot) to evaluate levels of pSTING, STING, pIRF3 and IRF3 and (E) densitometry quantification of pSTING/STING protein levels after 3 h cGAMP stimulation. Three independent experiments are plotted. (F) qPCR analysis of <t>Ifnb1</t> and Cxcl-10 expression was quantified relative to 18S mRNA levels after 6 h of cGAMP stimulation. BMDM cultures obtained from 6 individual mice (3 male and 3 female mice) are plotted. Bars indicate mean ± SEM and are normalized to cGAMP levels, expressed as a percentage. Statistical analyses were performed using Welch's t -test, yielding the following p values: For tissues: pTBK1: p = 0.0293, TBK1: p = 0.0283, Viperin: p = 0.0311, STAT1: p = 0.0159. For BMDMs: pSTING/STING: p = 0.0184, Ifnb1 : p < 0.0001, Cxcl-10 : *, p = 0.0367 and **, p = 0.0076. * Indicates p < 0.05, ** indicates p < 0.01, and **** indicates p < 0.0001.
Murine Embryonic Fibroblasts Nih 3t3 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fibroblast+migration+murine+fibroblasts/NIH%2F3T3/pmc02788845-301-1-9
Average 99 stars, based on 1 article reviews
murine embryonic fibroblasts nih 3t3 cells - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
murine l 2 cells  (ATCC)
99
ATCC murine l 2 cells
Intraperitoneal administration of nitroalkene compound CP-36 dampens STING-dependent inflammation in vivo. (A–C) Mice were administrated with CP-36 i.p. (10 or 30 mg/kg) 2 h before activating STING with i.p. injection of cAIM(PS) 2 Difluor (Rp/Sp) (0.2 mg/kg) for 4 h. Immunoblot analysis of homogenized and lysed (A) lung and (B) liver tissue to evaluate the levels of pTBK1, TBK1, pSTAT1, STAT1 and Viperin with (C) densitometry quantification of protein levels. The plots include data from individual mice represented in immunoblots. Bars indicate mean ± SEM and are normalized to the cAIM(PS) 2 Difluor (Rp/Sp) group, expressed as a percentage. (D–F) WT or STING-KO BMDMs were pre-treated with CP-36 (5 and 10 μM) or reduced CP-36 (R-CP-36, 5 and 10 μM) for 2 h, followed by cGAMP stimulation (4 μg/mL) for either 3 h or 6 h. (D) Immunoblot analysis of whole-cell lysate (representative blot) to evaluate levels of pSTING, STING, pIRF3 and IRF3 and (E) densitometry quantification of pSTING/STING protein levels after 3 h cGAMP stimulation. Three independent experiments are plotted. (F) qPCR analysis of <t>Ifnb1</t> and Cxcl-10 expression was quantified relative to 18S mRNA levels after 6 h of cGAMP stimulation. BMDM cultures obtained from 6 individual mice (3 male and 3 female mice) are plotted. Bars indicate mean ± SEM and are normalized to cGAMP levels, expressed as a percentage. Statistical analyses were performed using Welch's t -test, yielding the following p values: For tissues: pTBK1: p = 0.0293, TBK1: p = 0.0283, Viperin: p = 0.0311, STAT1: p = 0.0159. For BMDMs: pSTING/STING: p = 0.0184, Ifnb1 : p < 0.0001, Cxcl-10 : *, p = 0.0367 and **, p = 0.0076. * Indicates p < 0.05, ** indicates p < 0.01, and **** indicates p < 0.0001.
Murine L 2 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fibroblast+migration+murine+fibroblasts/L+Cells/pm38442151-249-0-11
Average 99 stars, based on 1 article reviews
murine l 2 cells - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
syf cells  (ATCC)
93
ATCC syf cells
Intraperitoneal administration of nitroalkene compound CP-36 dampens STING-dependent inflammation in vivo. (A–C) Mice were administrated with CP-36 i.p. (10 or 30 mg/kg) 2 h before activating STING with i.p. injection of cAIM(PS) 2 Difluor (Rp/Sp) (0.2 mg/kg) for 4 h. Immunoblot analysis of homogenized and lysed (A) lung and (B) liver tissue to evaluate the levels of pTBK1, TBK1, pSTAT1, STAT1 and Viperin with (C) densitometry quantification of protein levels. The plots include data from individual mice represented in immunoblots. Bars indicate mean ± SEM and are normalized to the cAIM(PS) 2 Difluor (Rp/Sp) group, expressed as a percentage. (D–F) WT or STING-KO BMDMs were pre-treated with CP-36 (5 and 10 μM) or reduced CP-36 (R-CP-36, 5 and 10 μM) for 2 h, followed by cGAMP stimulation (4 μg/mL) for either 3 h or 6 h. (D) Immunoblot analysis of whole-cell lysate (representative blot) to evaluate levels of pSTING, STING, pIRF3 and IRF3 and (E) densitometry quantification of pSTING/STING protein levels after 3 h cGAMP stimulation. Three independent experiments are plotted. (F) qPCR analysis of <t>Ifnb1</t> and Cxcl-10 expression was quantified relative to 18S mRNA levels after 6 h of cGAMP stimulation. BMDM cultures obtained from 6 individual mice (3 male and 3 female mice) are plotted. Bars indicate mean ± SEM and are normalized to cGAMP levels, expressed as a percentage. Statistical analyses were performed using Welch's t -test, yielding the following p values: For tissues: pTBK1: p = 0.0293, TBK1: p = 0.0283, Viperin: p = 0.0311, STAT1: p = 0.0159. For BMDMs: pSTING/STING: p = 0.0184, Ifnb1 : p < 0.0001, Cxcl-10 : *, p = 0.0367 and **, p = 0.0076. * Indicates p < 0.05, ** indicates p < 0.01, and **** indicates p < 0.0001.
Syf Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fibroblast+migration+murine+fibroblasts/SYF/pmc02660257-67-25-41
Average 93 stars, based on 1 article reviews
syf cells - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
murine fibroblast line 17cl 1 78 548  (ATCC)
99
ATCC murine fibroblast line 17cl 1 78 548
Intraperitoneal administration of nitroalkene compound CP-36 dampens STING-dependent inflammation in vivo. (A–C) Mice were administrated with CP-36 i.p. (10 or 30 mg/kg) 2 h before activating STING with i.p. injection of cAIM(PS) 2 Difluor (Rp/Sp) (0.2 mg/kg) for 4 h. Immunoblot analysis of homogenized and lysed (A) lung and (B) liver tissue to evaluate the levels of pTBK1, TBK1, pSTAT1, STAT1 and Viperin with (C) densitometry quantification of protein levels. The plots include data from individual mice represented in immunoblots. Bars indicate mean ± SEM and are normalized to the cAIM(PS) 2 Difluor (Rp/Sp) group, expressed as a percentage. (D–F) WT or STING-KO BMDMs were pre-treated with CP-36 (5 and 10 μM) or reduced CP-36 (R-CP-36, 5 and 10 μM) for 2 h, followed by cGAMP stimulation (4 μg/mL) for either 3 h or 6 h. (D) Immunoblot analysis of whole-cell lysate (representative blot) to evaluate levels of pSTING, STING, pIRF3 and IRF3 and (E) densitometry quantification of pSTING/STING protein levels after 3 h cGAMP stimulation. Three independent experiments are plotted. (F) qPCR analysis of <t>Ifnb1</t> and Cxcl-10 expression was quantified relative to 18S mRNA levels after 6 h of cGAMP stimulation. BMDM cultures obtained from 6 individual mice (3 male and 3 female mice) are plotted. Bars indicate mean ± SEM and are normalized to cGAMP levels, expressed as a percentage. Statistical analyses were performed using Welch's t -test, yielding the following p values: For tissues: pTBK1: p = 0.0293, TBK1: p = 0.0283, Viperin: p = 0.0311, STAT1: p = 0.0159. For BMDMs: pSTING/STING: p = 0.0184, Ifnb1 : p < 0.0001, Cxcl-10 : *, p = 0.0367 and **, p = 0.0076. * Indicates p < 0.05, ** indicates p < 0.01, and **** indicates p < 0.0001.
Murine Fibroblast Line 17cl 1 78 548, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fibroblast+migration+murine+fibroblasts/HeLa/10__1128_slash_jvi__00881___18-303-34-56
Average 99 stars, based on 1 article reviews
murine fibroblast line 17cl 1 78 548 - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
murine fibroblasts  (ATCC)
99
ATCC murine fibroblasts
Intraperitoneal administration of nitroalkene compound CP-36 dampens STING-dependent inflammation in vivo. (A–C) Mice were administrated with CP-36 i.p. (10 or 30 mg/kg) 2 h before activating STING with i.p. injection of cAIM(PS) 2 Difluor (Rp/Sp) (0.2 mg/kg) for 4 h. Immunoblot analysis of homogenized and lysed (A) lung and (B) liver tissue to evaluate the levels of pTBK1, TBK1, pSTAT1, STAT1 and Viperin with (C) densitometry quantification of protein levels. The plots include data from individual mice represented in immunoblots. Bars indicate mean ± SEM and are normalized to the cAIM(PS) 2 Difluor (Rp/Sp) group, expressed as a percentage. (D–F) WT or STING-KO BMDMs were pre-treated with CP-36 (5 and 10 μM) or reduced CP-36 (R-CP-36, 5 and 10 μM) for 2 h, followed by cGAMP stimulation (4 μg/mL) for either 3 h or 6 h. (D) Immunoblot analysis of whole-cell lysate (representative blot) to evaluate levels of pSTING, STING, pIRF3 and IRF3 and (E) densitometry quantification of pSTING/STING protein levels after 3 h cGAMP stimulation. Three independent experiments are plotted. (F) qPCR analysis of <t>Ifnb1</t> and Cxcl-10 expression was quantified relative to 18S mRNA levels after 6 h of cGAMP stimulation. BMDM cultures obtained from 6 individual mice (3 male and 3 female mice) are plotted. Bars indicate mean ± SEM and are normalized to cGAMP levels, expressed as a percentage. Statistical analyses were performed using Welch's t -test, yielding the following p values: For tissues: pTBK1: p = 0.0293, TBK1: p = 0.0283, Viperin: p = 0.0311, STAT1: p = 0.0159. For BMDMs: pSTING/STING: p = 0.0184, Ifnb1 : p < 0.0001, Cxcl-10 : *, p = 0.0367 and **, p = 0.0076. * Indicates p < 0.05, ** indicates p < 0.01, and **** indicates p < 0.0001.
Murine Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fibroblast+migration+murine+fibroblasts/NCTC+clone+929/10__1128_slash_iai__00866___08-55-0-3
Average 99 stars, based on 1 article reviews
murine fibroblasts - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
murine 3t3l1 preadipocytes  (ATCC)
99
ATCC murine 3t3l1 preadipocytes
Intraperitoneal administration of nitroalkene compound CP-36 dampens STING-dependent inflammation in vivo. (A–C) Mice were administrated with CP-36 i.p. (10 or 30 mg/kg) 2 h before activating STING with i.p. injection of cAIM(PS) 2 Difluor (Rp/Sp) (0.2 mg/kg) for 4 h. Immunoblot analysis of homogenized and lysed (A) lung and (B) liver tissue to evaluate the levels of pTBK1, TBK1, pSTAT1, STAT1 and Viperin with (C) densitometry quantification of protein levels. The plots include data from individual mice represented in immunoblots. Bars indicate mean ± SEM and are normalized to the cAIM(PS) 2 Difluor (Rp/Sp) group, expressed as a percentage. (D–F) WT or STING-KO BMDMs were pre-treated with CP-36 (5 and 10 μM) or reduced CP-36 (R-CP-36, 5 and 10 μM) for 2 h, followed by cGAMP stimulation (4 μg/mL) for either 3 h or 6 h. (D) Immunoblot analysis of whole-cell lysate (representative blot) to evaluate levels of pSTING, STING, pIRF3 and IRF3 and (E) densitometry quantification of pSTING/STING protein levels after 3 h cGAMP stimulation. Three independent experiments are plotted. (F) qPCR analysis of <t>Ifnb1</t> and Cxcl-10 expression was quantified relative to 18S mRNA levels after 6 h of cGAMP stimulation. BMDM cultures obtained from 6 individual mice (3 male and 3 female mice) are plotted. Bars indicate mean ± SEM and are normalized to cGAMP levels, expressed as a percentage. Statistical analyses were performed using Welch's t -test, yielding the following p values: For tissues: pTBK1: p = 0.0293, TBK1: p = 0.0283, Viperin: p = 0.0311, STAT1: p = 0.0159. For BMDMs: pSTING/STING: p = 0.0184, Ifnb1 : p < 0.0001, Cxcl-10 : *, p = 0.0367 and **, p = 0.0076. * Indicates p < 0.05, ** indicates p < 0.01, and **** indicates p < 0.0001.
Murine 3t3l1 Preadipocytes, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fibroblast+migration+murine+fibroblasts/3T3-L1/10__1074_slash_jbc__m204598200-76-0-3
Average 99 stars, based on 1 article reviews
murine 3t3l1 preadipocytes - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
murine 3t3-l1 fibroblasts  (ZenBio)
90
ZenBio murine 3t3-l1 fibroblasts
Intraperitoneal administration of nitroalkene compound CP-36 dampens STING-dependent inflammation in vivo. (A–C) Mice were administrated with CP-36 i.p. (10 or 30 mg/kg) 2 h before activating STING with i.p. injection of cAIM(PS) 2 Difluor (Rp/Sp) (0.2 mg/kg) for 4 h. Immunoblot analysis of homogenized and lysed (A) lung and (B) liver tissue to evaluate the levels of pTBK1, TBK1, pSTAT1, STAT1 and Viperin with (C) densitometry quantification of protein levels. The plots include data from individual mice represented in immunoblots. Bars indicate mean ± SEM and are normalized to the cAIM(PS) 2 Difluor (Rp/Sp) group, expressed as a percentage. (D–F) WT or STING-KO BMDMs were pre-treated with CP-36 (5 and 10 μM) or reduced CP-36 (R-CP-36, 5 and 10 μM) for 2 h, followed by cGAMP stimulation (4 μg/mL) for either 3 h or 6 h. (D) Immunoblot analysis of whole-cell lysate (representative blot) to evaluate levels of pSTING, STING, pIRF3 and IRF3 and (E) densitometry quantification of pSTING/STING protein levels after 3 h cGAMP stimulation. Three independent experiments are plotted. (F) qPCR analysis of <t>Ifnb1</t> and Cxcl-10 expression was quantified relative to 18S mRNA levels after 6 h of cGAMP stimulation. BMDM cultures obtained from 6 individual mice (3 male and 3 female mice) are plotted. Bars indicate mean ± SEM and are normalized to cGAMP levels, expressed as a percentage. Statistical analyses were performed using Welch's t -test, yielding the following p values: For tissues: pTBK1: p = 0.0293, TBK1: p = 0.0283, Viperin: p = 0.0311, STAT1: p = 0.0159. For BMDMs: pSTING/STING: p = 0.0184, Ifnb1 : p < 0.0001, Cxcl-10 : *, p = 0.0367 and **, p = 0.0076. * Indicates p < 0.05, ** indicates p < 0.01, and **** indicates p < 0.0001.
Murine 3t3 L1 Fibroblasts, supplied by ZenBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fibroblast+migration+murine+fibroblasts/3t3+l1+cells/pm26636414-35-4-11
Average 90 stars, based on 1 article reviews
murine 3t3-l1 fibroblasts - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


Intraperitoneal administration of nitroalkene compound CP-36 dampens STING-dependent inflammation in vivo. (A–C) Mice were administrated with CP-36 i.p. (10 or 30 mg/kg) 2 h before activating STING with i.p. injection of cAIM(PS) 2 Difluor (Rp/Sp) (0.2 mg/kg) for 4 h. Immunoblot analysis of homogenized and lysed (A) lung and (B) liver tissue to evaluate the levels of pTBK1, TBK1, pSTAT1, STAT1 and Viperin with (C) densitometry quantification of protein levels. The plots include data from individual mice represented in immunoblots. Bars indicate mean ± SEM and are normalized to the cAIM(PS) 2 Difluor (Rp/Sp) group, expressed as a percentage. (D–F) WT or STING-KO BMDMs were pre-treated with CP-36 (5 and 10 μM) or reduced CP-36 (R-CP-36, 5 and 10 μM) for 2 h, followed by cGAMP stimulation (4 μg/mL) for either 3 h or 6 h. (D) Immunoblot analysis of whole-cell lysate (representative blot) to evaluate levels of pSTING, STING, pIRF3 and IRF3 and (E) densitometry quantification of pSTING/STING protein levels after 3 h cGAMP stimulation. Three independent experiments are plotted. (F) qPCR analysis of Ifnb1 and Cxcl-10 expression was quantified relative to 18S mRNA levels after 6 h of cGAMP stimulation. BMDM cultures obtained from 6 individual mice (3 male and 3 female mice) are plotted. Bars indicate mean ± SEM and are normalized to cGAMP levels, expressed as a percentage. Statistical analyses were performed using Welch's t -test, yielding the following p values: For tissues: pTBK1: p = 0.0293, TBK1: p = 0.0283, Viperin: p = 0.0311, STAT1: p = 0.0159. For BMDMs: pSTING/STING: p = 0.0184, Ifnb1 : p < 0.0001, Cxcl-10 : *, p = 0.0367 and **, p = 0.0076. * Indicates p < 0.05, ** indicates p < 0.01, and **** indicates p < 0.0001.

Journal: Redox Biology

Article Title: Development of nitroalkene-based inhibitors to target STING-dependent inflammation

doi: 10.1016/j.redox.2024.103202

Figure Lengend Snippet: Intraperitoneal administration of nitroalkene compound CP-36 dampens STING-dependent inflammation in vivo. (A–C) Mice were administrated with CP-36 i.p. (10 or 30 mg/kg) 2 h before activating STING with i.p. injection of cAIM(PS) 2 Difluor (Rp/Sp) (0.2 mg/kg) for 4 h. Immunoblot analysis of homogenized and lysed (A) lung and (B) liver tissue to evaluate the levels of pTBK1, TBK1, pSTAT1, STAT1 and Viperin with (C) densitometry quantification of protein levels. The plots include data from individual mice represented in immunoblots. Bars indicate mean ± SEM and are normalized to the cAIM(PS) 2 Difluor (Rp/Sp) group, expressed as a percentage. (D–F) WT or STING-KO BMDMs were pre-treated with CP-36 (5 and 10 μM) or reduced CP-36 (R-CP-36, 5 and 10 μM) for 2 h, followed by cGAMP stimulation (4 μg/mL) for either 3 h or 6 h. (D) Immunoblot analysis of whole-cell lysate (representative blot) to evaluate levels of pSTING, STING, pIRF3 and IRF3 and (E) densitometry quantification of pSTING/STING protein levels after 3 h cGAMP stimulation. Three independent experiments are plotted. (F) qPCR analysis of Ifnb1 and Cxcl-10 expression was quantified relative to 18S mRNA levels after 6 h of cGAMP stimulation. BMDM cultures obtained from 6 individual mice (3 male and 3 female mice) are plotted. Bars indicate mean ± SEM and are normalized to cGAMP levels, expressed as a percentage. Statistical analyses were performed using Welch's t -test, yielding the following p values: For tissues: pTBK1: p = 0.0293, TBK1: p = 0.0283, Viperin: p = 0.0311, STAT1: p = 0.0159. For BMDMs: pSTING/STING: p = 0.0184, Ifnb1 : p < 0.0001, Cxcl-10 : *, p = 0.0367 and **, p = 0.0076. * Indicates p < 0.05, ** indicates p < 0.01, and **** indicates p < 0.0001.

Article Snippet: RNA levels for murine Ifnb1 (Mm00439552_s1) and Actb (Mm02619580_g1) were analyzed using premade TaqMan assays and the RNA-to-Ct-1-Step kit according to manufacturer's recommendations.

Techniques: In Vivo, Injection, Western Blot, Expressing